Increasing Scopolamine content in Hairy Roots of Atropa belladonna using Bioreactor
نویسندگان
چکیده
منابع مشابه
Promoting scopolamine accumulation in transgenic plants of Atropa belladonna generated from hairy roots with over expression of pmt and h6h gene
Laboratory of Natural Products and Metabolic Engineering, Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Sciences, Southwest University, Chongqing 400715, People’s Republic of China. School of Pharmaceutical Sciences, Southwest University, Chongqing 400715, People’s Republic of China. Institute of Ornamental Plants Research, Southwest...
متن کاملDifferential production of tropane alkaloids in hairy roots and in vitro cultured two accessions of Atropa belladonna L. under nitrate treatments.
Plants are a potential source of a large number of valuable secondary metabolites. In vitro cultures are being considered as an alternative to agricultural processes for studying valuable secondary metabolites. In this way, nutritive factors are important parameters influencing the production of these compounds in plants. Effects of nitrate concentrations (KNO3) on the production of two tropane...
متن کاملAtropa Belladonna intoxication: a case report
Atropa belladonna is a poisonous plant also called deadly nightshade. Its roots, leaves and fruits contain alkaloids: atropine, hyocyamine and scopolamine. The risk of poisoning in children is important because of possible confusion with other berries. Atropa belladonna acute intoxication is a severe condition, it's should be considered in the presence of anti-cholinergic toxidrome, the differe...
متن کاملImprovement of tropane alkaloids production in hairy root cultures of Atropa belladonna by overexpressing pmt and h6h genes
Atropa belladonna L. is the commercial plant to produce tropane alkaloids including hyoscyamine and scopolamine, which are widely used as anticholinergic agents. In the present study, the genes encoding the key enzymes including putrescine N-methyltransferase (PMT) and hyoscyamine 6β-hydroxylase (H6H) were simultaneously overexpressed in transgenic hairy root cultures of A. belladonna. All of t...
متن کاملEnhancement of Tropane Alkaloid Production among Several Clones and Explants Types of Hairy Root of Atropa belladonna L.
Agrobacterium rhizogenes (pRi), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of plant species. In our study four bacterial strains AR15834, A4, 9435 and C318 and three explants types leaf, stems and roots, were examined. Hairy roots were induced from roots, stems and leaf explants. The highest transformation efficiency of 77% was achieved by usi...
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ژورنال
عنوان ژورنال: Brazilian Archives of Biology and Technology
سال: 2015
ISSN: 1516-8913
DOI: 10.1590/s1516-8913201400276